Qantas has cut direct flights from Canberra to Darwin, citing a lack of demand.
The airline announced the decision on Monday afternoon, as one of several changes to its flights into and out of the Northern Territory.
The Canberra to Darwin route was seasonal, and the airline flew up to three direct flights a week.
The changes will force Canberrans to travel indirectly to Darwin, either through Sydney, Adelaide or Brisbane.
The direct route will stop in April.
The Capital Airport Group declined to comment, saying it does not discuss the operational decisions of individual airlines.
Qantas also announced it would stop flying from Sydney to Uluru.
Jetstar will now offer four flights a week on its Airbus A320 aircraft from Tuesday, June 4.
Qantas said in a statement the Sydney to Uluru route had not been profitable in years, and flights regularly left with up to 40per cent of seats empty.
The route changes to the Top End came after independent MP Bob Katter announced he had drafted new laws that would only allow Qantas access to domestic flights and ports if it met a legal definition of being "Australian".
That definition includes that the company be 51 per cent owned by Australian citizens or residents, that at least 80 per cent of its maintenance be done here and that all airline crews live in Australia. with AAP