Virgin Australia will indefinitely ban boxers, bulldogs and pugs from its flights following the death of two dogs in transit last month.
The new rules, which will come into effect on Friday, follow Qantas' decision last week to temporarily halt flights for snub-nosed breeds after two dogs being transported by the airline died in separate incidents.
Snub-nosed dogs, which include pugs, boxers and bulldogs, often suffer from obstructed airways, making them prone to respiratory problems and making it more difficult for them to withstand extreme heat.
The RSPCA said it was "simply too dangerous" to transport snub-nosed dogs, even with the highest level of care.
Qantas passenger Kay Newman accused the airline of negligence after her boxer died while being transported from Sydney to Brisbane on December 19.
Ms Newman said her dog Duke was left on the tarmac with no shade in 39-degree heat for more than an hour before the flight.
Two days later, a bulldog named Frank died on a flight from Sydney to Melbourne.
Virgin will honour all existing flight bookings for snub-nosed dogs but no bookings to transport these breeds will be accepted from Friday, with the ban set to remain in place indefinitely.
Customers will be given refunds if they choose to cancel existing bookings for snub-nosed dogs.
A spokesman for Virgin Australia said the airline was working with the RSPCA to improve procedures for transporting animals.
"We have seen a tragic spike in issues across the industry when transporting snub-nosed breeds and this has prompted Virgin Australia to review the transport of these animals," he said.
"While we understand that snub-nosed breeds are a popular choice of pet, they are a high-risk animal to transport due to their known respiratory issues and other health problems that may be compounded in-flight."
"Virgin Australia has made the difficult decision to no longer accept bookings for snub-nosed breeds until further notice."
The airline already has a policy of urging passengers not to fly with snub-nosed dogs and requiring those who do to sign a form agreeing they accept responsibility for any "challenges" the pet faces on the flight.
The RSPCA welcomed the airline's ban but warned snub-nosed dogs were not physically equipped to fly on planes in any circumstance.
"The RSPCA believes the inherent risks to these flat-faced breeds are unfortunately so great, that even with the best care, we believe transporting them by air is simply too dangerous," said the spokeswoman.
"We would urge owners to avoid flying these breeds at all if possible, and if air travel is considered absolutely necessary, owners should speak to their vet and be very aware of thedangers."